By Peter Malcolm Wallis, Brian R. Hammond
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At higher magnification, seen in Figure 7, the contrast between the flagellated trophozoite and the in vitro formed cyst was readily apparent. The cysts were oval to round in shape and possessed a smooth surface, whereas the pearshaped trophozoites were flattened in a dorsalventral plane and attached to the substratum by their ventral adhesive disc. Bar equals 5 microns for Figure 6 and 1 micron for Figure 7. By day three, these clusters had increased in size and included trophozoites as well as cysts.
Giemsa × 400. lamblia monolayer (87 h). lamblia were similar. Interaction Between Vero and HeLa Cells and the Culture Supernatant No morphological changes were seen in either HeLa or Vero cells from each of the four strains upon incubation with the culture supernatant up to a period of 96 h compared to the matched controls. lamblia extracts apparently had no deleterious effects and did not induce any morphological changes compared with TPS1 and MEM control culture cells in either HeLa or Vero cells till 96 h after interaction.
69104. Paris 136: 67 70. 40:821832. Biophysical and Biochemical Cytology 9:409413. 2:187196. 74:429433. 13:1618. Gastroenterology 71:5761. 53:704707. J. 73:723729. Gastroenterology, In press. 921. lamblia trophozoites. These observations suggest that direct cell to parasite contact may play an important role in damaging the host cells. One reported the significance of bile salt deconjugation by the parasite (12) and the other the cytopathogenic effects of Giardia on cell monolayers, in vitro (11).
Advances in Giardia Research by Peter Malcolm Wallis, Brian R. Hammond