New PDF release: Antiphospholipid Syndrome

By A. Bulikova

ISBN-10: 9535105264

ISBN-13: 9789535105268

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Additional info for Antiphospholipid Syndrome

Sample text

It is likely, that also these observations were obtained with the open ‘activated’ conformation of β2GPI. The purification method that all these groups used included the use of perchloric acid, and these harsh conditions may have induced a conformational change of closed native β2GPI into the open activated conformation of β2GPI. It is highly recommended that the conformation of β2GPI is confirmed in coagulation tests or by electron microscopy. It is expected that in the near future specific immunological assays will become available to determine the specific conformational state of β2GPI.

It goes beyond saying that the appreciation of the different conformations and the ability to switch conformation has provided an important basis for research in this area. 4. Acknowledgments This research was supported in part by a grant from the Netherlands Organization for Scientific Research (ZonMW 91207002; JCM Meijers and PG de Groot) and an Academic Medical Center stimulation grant (JCM Meijers). Structural Changes in β2-Glycoprotein I and the Antiphospholipid Syndrome 31 5. References Abdel-Monem H, Dasgupta SK, Le A, Prakasam A & Thiagarajan P.

2010). Blood contains microparticles (MPs) derived from a variety of cell types, including platelets, monocytes, and endothelial cells. MPs are formed from membrane blebs that are released from the cell surface by proteolytic cleavage of the cytoskeleton (Owens & Mackman, 2011). MPs may be procoagulant because they provide a membrane surface for the assembly of components of the coagulation protease cascade. Importantly, 30 Antiphospholipid Syndrome procoagulant activity is increased by the presence of anionic phospholipids, particularly phosphatidylserine (PS), and the procoagulant protein tissue factor (TF), which is the major cellular activator of the clotting cascade (Owens & Mackman, 2011).

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Antiphospholipid Syndrome by A. Bulikova

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